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BISPECIFICS
WEBINAR
EFFICIENT PRODUCTION OF BISPECIFIC MOLECULES 

Streamlined Development for Efficient Production of Bispecific Molecules using an Integrated Platform Process

 
High-quality clinical bulk drug substances under accelerated timelines.
That's the power you have with KBI and Selexis.
That's the power of together.
  selexis-kbi

BISPECIFICS WEBINAR

CATCH THE HIGHLIGHTs

There is a global need for high quality, effective off-the-shelf therapies that can be used immediately, while concurrently avoiding supply chain issues and mitigating process complexities. Bispecific antibodies (bsAbs) are constructed to help meet this need due to their ability to bind to two or more different epitopes, thereby allowing them to perform multiple discrete mechanisms of action.

This webinar will demonstrate a breakthrough platform approach that encompasses the efficient production of bispecific molecules in an integrated, streamlined way, from CLD (cell line development) to cGMP manufacturing. The leverageable integrated workflow from Selexis and KBI Biopharma generates high quality clinical bulk drug substances under accelerated timelines.

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Highlights from the Bispecifics Webinar:

 

  • Selexis uses an industry-leading CLD platform that utilizes a mammalian single-cell system to express any bispecific antibody format. The platform has been adapted to this specific class of molecules. Separate vectors, each harboring a different polypeptide chain from a bsAb, are adjusted to favor bias toward heterodimer production. Isolation of monoclonal cell lines is based on high expressing clones, including heterodimer purity.

  • KBI begins process development efforts, prior to the generation of single-cell clones, using early transfection pools generated by Selexis. Upstream and downstream process development studies occur in parallel with analytical and pre-formulation development activities, greatly shortening the overall development timeline.

  • The Selexis-KBI partnership offers far more beyond the combination of best-in-class CLD with unparalleled expertise and experience in mammalian contract development and manufacturing. These highly productive “client-to-industry” collaborations prioritize quality, efficiencies, and time savings in bsAb production across a diverse portfolio of molecule formats to strengthen client programs.

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Extensive Q&A

ANSWERING your QUESTIONS

Your Questions, Answered.

Webinar registrants and participants were invited to submit their questions both before the webinar and during the live event. Questions addressed include: 

  • Do you have a proprietary CHO cell line that is used for transfections?
  • Are some cell lines better for specific format bispecifics? 
  • For the transfection of 2HC and 1LC, do you use 3 different selections for the 3 vectors?
  • Could you describe some of the molecules you have worked with where an additional domain/molecule was appended to the Fc domain - any challenges associated with expressing those molecules?
  • How do you determine the copy number of your plasmid in the cells? How do you control this parameter as it for sure has an influence on the performance of your winner clone?
  • How do you address cells and best clone selection? Are you using FACS, MACS Quant Tyto, Berkeley Light, etc?
  • How well does process understanding and improvement using early candidate pools translate to the final clone? 
  • How many different types of pairing technologies have you worked with?
  • How do Selexis and KBI coordinate project activities with one another?
  • Are you developing any bsAbs with a payload? If so, what types of payloads (radioactive, toxic, etc)?
  • What is your experience using cell lines that produce afucosylated mAbs for enhanced ADCC?
  • Are development timelines of bispecific projects similar to mAb projects?

Ready to Learn More?
Access the Bispecific Webinar!